Crystallization (Comment) | Organism |
---|---|
purified recombinant AtSOT18 in complex with PAP alone and together with the glycosinolate sinigrin, X-ray diffraction structure determination and analysis at 1.74-1.92 A resolution | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
H155A | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
K93A | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
P136A | site-directed mutagenesis, the mutant shows unaltered activity compared to wild-type enzyme | Arabidopsis thaliana |
T96A | site-directed mutagenesis, mutant Thr96Ala shows residual activity with the preferred substrates 3-methylthiopropyl desulfoglucosinolate and 8-methylthiooctyl desulfoglucosinolate (12fold reduction and 3fold reduction, respectively), while with sinigrin no activity is detected | Arabidopsis thaliana |
T97A | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
Y130A | site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 3',5'-bisphosphate | PAP, competitive inhibition | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + desulfosinigrin | Arabidopsis thaliana | - |
adenosine 3',5'-bisphosphate + sinigrin | - |
? | |
3'-phosphoadenylyl sulfate + desulfosinigrin | Arabidopsis thaliana Col-0 | - |
adenosine 3',5'-bisphosphate + sinigrin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9C9C9 | - |
- |
Arabidopsis thaliana Col-0 | Q9C9C9 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0073 | - |
recombinant mutant T96A, substrate 3-methylthiopropyl desulfoglucosinolate, pH 8.0, 37°C | Arabidopsis thaliana |
0.028 | - |
recombinant mutant P136A, substrate glucobrassicin, pH 8.0, 37°C | Arabidopsis thaliana |
0.03 | - |
recombinant wild-type enzyme, substrate glucobrassicin, pH 8.0, 37°C | Arabidopsis thaliana |
0.032 | - |
recombinant mutant T96A, substrate 8-methylthiooctyl desulfoglucosinolate, pH 8.0, 37°C | Arabidopsis thaliana |
0.053 | - |
recombinant wild-type enzyme, substrate sinigrin, pH 8.0, 37°C | Arabidopsis thaliana |
0.0971 | - |
recombinant wild-type enzyme, substrate 8-methylthiooctyl desulfoglucosinolate, pH 8.0, 37°C | Arabidopsis thaliana |
0.0974 | - |
recombinant wild-type enzyme, substrate 3-methylthiopropyl desulfoglucosinolate, pH 8.0, 37°C | Arabidopsis thaliana |
0.104 | - |
recombinant mutant P136A, substrate 3-methylthiopropyl desulfoglucosinolate, pH 8.0, 37°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + 3-methylthiopropyl desulfoglucosinolate | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 3-methylthiopropyl glucosinolate | - |
? | |
3'-phosphoadenylyl sulfate + 3-methylthiopropyl desulfoglucosinolate | - |
Arabidopsis thaliana Col-0 | adenosine 3',5'-bisphosphate + 3-methylthiopropyl glucosinolate | - |
? | |
3'-phosphoadenylyl sulfate + desulfosinigrin | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + sinigrin | - |
? | |
3'-phosphoadenylyl sulfate + desulfosinigrin | - |
Arabidopsis thaliana Col-0 | adenosine 3',5'-bisphosphate + sinigrin | - |
? | |
adenosine 3',5'-bisphosphate + 7-methylthioheptyl glucosinolate | - |
Arabidopsis thaliana | 3'-phosphoadenylyl sulfate + 7-methylthioheptyl desulfoglucosinolate | - |
? | |
adenosine 3',5'-bisphosphate + 7-methylthioheptyl glucosinolate | - |
Arabidopsis thaliana Col-0 | 3'-phosphoadenylyl sulfate + 7-methylthioheptyl desulfoglucosinolate | - |
? | |
adenosine 3',5'-bisphosphate + 8-methylthiooctyl glucosinolate | - |
Arabidopsis thaliana | 3'-phosphoadenylyl sulfate + 8-methylthiooctyl desulfoglucosinolate | - |
? | |
adenosine 3',5'-bisphosphate + 8-methylthiooctyl glucosinolate | - |
Arabidopsis thaliana Col-0 | 3'-phosphoadenylyl sulfate + 8-methylthiooctyl desulfoglucosinolate | - |
? | |
adenosine 3',5'-bisphosphate + sinigrin | sinigrin binding is facilitated by hydrogen bonding with residues Arg51, Glu54, Thr96, Tyr130, His155 and Tyr306. The guanidinium group of Arg51 interacts with the 6'-hydroxyl group of the glucopyranose of the glucosinolate. The carboxyl group of Glu54 interacts with the 4'- and the 6'-hydroxyl groups. The hydroxyl of Tyr306 forms a hydrogen bond with the oxygen in the glucopyranose ring. The sulfate moiety is stabilized by hydrogen bonds with Thr96, Tyr130 and His155. In this positioning the sulfate and phosphate groups of sinigrin and PAP, respectively, are located within the hydrogen bond distance from each other. Analysis of the mechanism of PAPS binding | Arabidopsis thaliana | 3'-phosphoadenylyl sulfate + desulfosinigrin | - |
? | |
additional information | enzyme AtSOT18 prefers the long-chain ds-Gls 7-methylthioheptyl Gl and 8-methylthiooctyl Gl (8MTO), that are also derived from methionine | Arabidopsis thaliana | ? | - |
- |
|
additional information | enzyme AtSOT18 prefers the long-chain ds-Gls 7-methylthioheptyl Gl and 8-methylthiooctyl Gl (8MTO), that are also derived from methionine | Arabidopsis thaliana Col-0 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
More | AtSOT18 does not contain the dimerization motif KxxxTVxxxE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase | UniProt | Arabidopsis thaliana |
AtSOT18 | - |
Arabidopsis thaliana |
cytosolic sulfotransferase 18 | - |
Arabidopsis thaliana |
desulpho-Gl SOT | - |
Arabidopsis thaliana |
ds-Gl SOT | - |
Arabidopsis thaliana |
SOT18 | - |
Arabidopsis thaliana |
sulphotransferase 18 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
purified enzyme, 10 min, pH 8.0, inactivation | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | sulfotransferases (SOTs or SULTs) can be found in all organisms analysed so far. In Arabidopsis thaliana, twenty-two sulfotransferase (SOT) isoforms are identified. Three of those are involved in glucosinolate (Gl) biosynthesis, glycosylated sulfur-containing aldoximes containing chemically different side chains, whose breakdown products are involved in stress response against herbivores, pathogens, and abiotic stress. The structure of AtSOT18 invigorates the similarity between plant and mammalian sulfotransferases, which illustrates the evolutionary conservation of this multifunctional enzyme family. In Arabidopsis thaliana, the three SOTs AtSOT16, AtSOT17 and AtSOT18 exclusively catalyse the transfer of a sulfuryl group to different ds-Gls. All three enzymes are localized in the cytoplasm and their expression pattern tested under several conditions is similar. They show 72% sequence identity but differ remarkably in their substrate specificity | Arabidopsis thaliana |
additional information | structure homology modeling | Arabidopsis thaliana |
physiological function | sulfotransferases catalyse the transfer of a sulfuryl group from the co-substrate 3'-phospho-adenosine 5'-phosphosulfate (PAPS) to a hydroxyl group of various substrates. In plants, sulfated compounds act as hormones, secondary metabolites in stress defense and probably serve as a reservoir for sulfur | Arabidopsis thaliana |